Coding

Part:BBa_K3937002:Design

Designed by: Xuanshuo Zhang   Group: iGEM21_NPU-CHINA   (2021-09-30)


new-BADE->aflatoxin


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal PstI site found at 433
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal PstI site found at 433
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 18
    Illegal XhoI site found at 871
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal PstI site found at 433
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal PstI site found at 433
    Illegal AgeI site found at 190
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

This sequence is codon optimized to codon usage bias of E. coli. Also, we recommend seamless cloning to assemble the parts.


Source

BADE is from Bacillus subtilis 168 genome.

References